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In an extended 10 day oral and contact bee screen test, 2 days old young worker bees (Apis mellifera) were exposed once to one dose rate of the lead construct and a control via an oral test (50% sucrose solution) and a contact test (thoracic application). During this experiment food consumption, bee mortality and behavioural effects were assessed daily. There were 3 replicates per dose rate with 10 bees/cage. The test conditions were 33±2°C and 50-70% relative humidity. No CO2 - anaesthetization was used as young bees are easy to handle.

During the oral application the bees were fed once with treated 50% (w/v) sucrose solution. During the contact application droplets were placed on the dorsal honeybee thorax.

During the test after application, honeybees were provided continuously with untreated sucrose solution via syringes. Food consumption was assessed every 24 hours. Therefore syringes were weighed before and after administration to the bees. Syringes were replaced daily.

Cumulative mortality was calculated as the mean mortality of all the replicates per day. Consumed sucrose solution was calculated by {amount of sucrose solution lost from the syringe per day/(number of alive bees)}. Behavioural abnormalities was given by number of bees exhibiting symptoms per day.

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The Resource ID for this resource is d931d13d-c733-472b-b52f-24dd505b56a7.

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filesize3.15 KB
resource typefile upload
timestampJun 23, 2017
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