{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"f9a1dfec-7088-44f0-b386-1f25b478fabe","name":"beneficials-description","title":"Beneficials Description","maintainer":"Syngenta","maintainer_email":"katsivelis.panagis@agroknow.com","notes":"\u003Cp\u003EThe \u0022RNA-based biocontrols Open Data - Beneficials Description\u0022 datasets include biological screening data for the lead RNA-based biocontrol candidate for the Colorado Potato Beetle tested for mortality against common non-target beneficial species (bees and parasitic\/predatory insects).\u003C\/p\u003E\n","url":"https:\/\/opendata.syngenta.agroknow.com\/dataset\/beneficials-description","state":"Active","log_message":"Edited by stoitsis.","private":true,"revision_timestamp":"Mon, 05\/16\/2022 - 14:32","metadata_created":"Thu, 05\/18\/2017 - 13:03","metadata_modified":"Mon, 05\/16\/2022 - 14:32","creator_user_id":"d758bd40-12c2-4095-8e0a-1dd7125d98bd","type":"Dataset","resources":[{"id":"fbf6238a-5975-4d10-aaa7-5a1eb015b56d","revision_id":"","url":"https:\/\/opendata.syngenta.agroknow.com\/sites\/default\/files\/Aphidius_colemani_Orius_laevigatus_Typhlodromus_pyri_v1.1_0.csv","description":"\u003Cp\u003EThis dataset is the result of a study to assess the effect of the RNA-based biocontrol candidate for Colorado Potato Beetle on a selection of beneficial species \u003Cem\u003EAphidius colemani, Orius laevigatus, Typhlodromus pyri\u003C\/em\u003E.\u003C\/p\u003E\n\u003Cp\u003EThe treatment object for this experiment was the bushbean plant (\u003Cem\u003EPhaseolus vulgaris\u003C\/em\u003E). Each bushbean plant was reduced to 1 leaf. 6 bushbean plants at a time were then sprayed with compound solution of 1x, 2x and 4x the field rate and left to dry. Plants were treated in a spray application box where the nozzle moves 1 time horizontally over the plants and left to dry; 1 hour later the bushbean plants were returned to a greenhouse chamber at 20\u00b0C and 60 % relative humidity.\u003C\/p\u003E\n\u003Cp\u003EThe bioassays were setup and rated using Syngenta\u2019s standard bioassay screening procedures, although this was not a GLP experiment.\u003C\/p\u003E\n\u003Ch3\u003E\u003Cem\u003EAphidius colemani\u003C\/em\u003E  Set Up\u003C\/h3\u003E\n\u003Cp\u003EThe same day plastic dishes were prepared with 5 ml of water agar 1 %. A leaf disc was punched from each bushbean plant with a 5 cm diameter and stuck upper side up in the plastic dish. Each plastic dish was closed using a lid with a 0.5 cm diameter hole.\u003C\/p\u003E\n\u003Cp\u003E5 \u003Cem\u003EAphidius colemani\u003C\/em\u003E adults were added to each plastic dish. The plastic dishes were then covered up with a cotton filter soaked with a 20 % sucrose solution.\u003C\/p\u003E\n\u003Cp\u003EThe plastic dishes were incubated for 2 and 4 days in a climate chamber at 21\u00b0C and 75 % RH, upper side down. 2 and 4 days after infestation the living and dead \u003Cem\u003EAphidius colemani\u003C\/em\u003Ewere counted.\u003C\/p\u003E\n\u003Ch3\u003E\u003Cem\u003EOrius laevigatus\u003C\/em\u003E Set Up\u003C\/h3\u003E\n\u003Cp\u003EThe same day plastic dishes were prepared with FLUON 100 % and 5 ml of water agar 1 %. A leaf disc was punched from each bushbean plant with a 5cm diameter each and stuck upper side up in the plastic dish, using insect glue around the border of each leaf disc. Some \u003Cem\u003EEphestia kuehniella\u003C\/em\u003E eggs and a paper triangle were added to each disc.\u003C\/p\u003E\n\u003Cp\u003E5 \u003Cem\u003EOrius laevigatus\u003C\/em\u003E adults were added to each plastic dish. The plastic dishes were then covered up with a cotton filter and a lid with three holes of 0.5 cm diameter.\u003C\/p\u003E\n\u003Cp\u003EThe plastic dishes were incubated for 2 and 4 days in a climate chamber at 25\u00b0C and 75 % RH, upper side up. 2 and 4 days after infestation the living and dead \u003Cem\u003EOrius laevigatus\u003C\/em\u003E were counted.\u003C\/p\u003E\n\u003Ch3\u003E\u003Cem\u003ETyphlodromus pyri\u003C\/em\u003E Set Up\u003C\/h3\u003E\n\u003Cp\u003EThe same day plastic dishes were prepared with a 5 cm diameter cotton pad and 9ml of water. A leaf disc was punched from each bushbean plant with a 3.5 cm diameter and stuck upper side up in the plastic dish, using insect glue around the border of each leaf disc.\u003C\/p\u003E\n\u003Cp\u003EUsing forceps, a small piece of cotton wool was pulled through these leaf discs. 10\u003Cem\u003ETyphlodromus pyri\u003C\/em\u003E protonymphs were added to each plastic dish and some apple pollen. The plastic dishes were then covered up with a cotton filter and a lid with three holes of 0.5 cm diameter.\u003C\/p\u003E\n\u003Cp\u003EThe plastic dishes were incubated for 3 and 7 days in a climate chamber at 27\u00b0C and 40 % relative humidity, upper side up. 3 and 7 days after infestation the living and dead \u003Cem\u003ETyphlodromus pyri\u003C\/em\u003E were counted.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Mon, 09\/16\/2019 - 14:42","name":"Aphidius colemani, Orius laevigatus, Typhlodromus pyri ","mimetype":"text\/csv","size":"39 KB","created":"Thu, 05\/18\/2017 - 13:04","resource_group_id":"","last_modified":"Date changed  Mon, 09\/16\/2019 - 14:42"},{"id":"d931d13d-c733-472b-b52f-24dd505b56a7","revision_id":"","url":"https:\/\/opendata.syngenta.agroknow.com\/sites\/default\/files\/Honeybee_v1.1_0.csv","description":"\u003Cp\u003EIn an extended 10-day oral and contact bee screening test, 2-day old young worker bees (Apis mellifera) were exposed to a single dose rate of the lead construct, a media control or an untreated sucrose control via an oral test (50% sucrose solution) and a contact test (thoracic application). The concentration in the dose was approximately 10x the concentration applied in the field. During this experiment food consumption, bee mortality and any behavioural effects were assessed daily. There were 3 replicates with 10 bees\/cage. There were 3 replicates per dose rate with 10 bees\/cage. The test conditions were 33\u00b12\u00b0C and 50-70% relative humidity. No CO2 - anaesthetization was used as young bees are easy to handle.\u003C\/p\u003E\n\u003Cp\u003EDuring the oral application the bees were fed for the first 24 hrs with the treated or control 50% (w\/v) sucrose solution. During the contact application droplets were placed on the dorsal honeybee thorax.\u003C\/p\u003E\n\u003Cp\u003EAfter dosing honeybees were provided continuously with untreated sucrose solution via syringes. Food consumption was assessed every 24 hours. Therefore syringes were weighed before and after administration to the bees. Syringes were replaced daily.\u003C\/p\u003E\n\u003Cp\u003ECumulative mortality was calculated as the mean mortality of all the replicates per day. Consumed sucrose solution was calculated by {amount of sucrose solution lost from the syringe per day\/(number of alive bees)}. Behavioural abnormalities was given by number of bees exhibiting symptoms per day.\u003C\/p\u003E\n","format":"csv","state":"Active","revision_timestamp":"Mon, 09\/16\/2019 - 14:46","name":"Honeybee","mimetype":"text\/csv","size":"3.14 KB","created":"Thu, 05\/18\/2017 - 13:06","resource_group_id":"","last_modified":"Date changed  Mon, 09\/16\/2019 - 14:46"},{"id":"7040ecb0-f409-45ff-8263-e30d57821297","revision_id":"","url":"https:\/\/opendata.syngenta.agroknow.com\/sites\/default\/files\/Aphidius_colemani_Orius_laevigatus_Typhlodromus_pyri.ttl","description":"","format":"ttl","state":"Active","revision_timestamp":"Mon, 06\/26\/2017 - 14:23","name":"Aphidius colemani, Orius laevigatus, Typhlodromus pyri - RDF Version","mimetype":"application\/octet-stream","size":"154.97 KB","created":"Tue, 06\/20\/2017 - 10:50","resource_group_id":"","last_modified":"Date changed  Mon, 06\/26\/2017 - 14:23"},{"id":"1f2e7e71-1992-4d58-b4bb-ef22b37a24ff","revision_id":"","url":"https:\/\/opendata.syngenta.agroknow.com\/sites\/default\/files\/honeybee_0.ttl","description":"","format":"ttl","state":"Active","revision_timestamp":"Mon, 06\/26\/2017 - 14:44","name":"Honeybee - RDF Version","mimetype":"application\/octet-stream","size":"65.1 KB","created":"Tue, 06\/20\/2017 - 10:51","resource_group_id":"","last_modified":"Date changed  Mon, 06\/26\/2017 - 14:44"}]}]}